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Neves,M.M.; Marques Jr.,A.P.; Santana,C.V.; Lima,F.P.C.; Zambrano,W.J.. |
The ovarian characteristics of pregnant and non pregnant zebu cows (Bos taurus indicus) were studied. The ovaries were obtained in slaughterhouses, identified and divided into: group I, non pregnant animals (GI, n=43) and group II, pregnant animals (GII, n=43). The ovaries were measured and cut in their lenght for measuring and evaluation of the corpus luteum (CL) characteristics. For the GI the measures for the left ovary were 2.5 ± 0.70, 1.61 ± 0.32 and 1.22 ± 0.39cm, respectively for length, width and thickness, and 2.62 ± 0.54, 1.71 ± 0.36 and 1.21 ± 0.31cm for the right ovary. For the GII the measures for the left ovary were 2.78 ± 0.48, 1.80 ± 0.33 and 1.23 ± 0.35cm, respectively for length, width and thickness and 2.84 ± 0.50, 1.74 ± 0.45 and 1.21 ±... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; Zebu; Ovário; Corpo lúteo. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000600016 |
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Neves,M.M.; Heneine,L.G.D.; Henry,M.. |
The aim of this study was to evaluate the use of low concentrations of natural and lyophilized low density lipoprotein (LDL) from hen's egg yolk for cryopreservation of canine semen. Different ammonium sulphate concentrations were tested to extract LDL from egg yolk. The yolk was centrifuged, and LDL was isolated using 10, 20, 40, 45, or 50% ammonium sulphate solution (ASS). The LDL-rich floating fraction was collected for chemical characterization. Dry matter content was lowest (P<0.05) in the LDL extracted with the 50% ASS. The purification of LDL increased in association with increasing ammonium sulphate concentrations. SDS-PAGE showed that the 50% ASS solution yielded a purer fraction of LDL from egg yolk. For semen cryopreservation, TRIS extender... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Egg Yolk; LDL extraction; Ammonium sulphate; Dog; Semen. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300769 |
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Morais,D.B.; Barbosa,L.P.; Melo,B.E.S.; Matta,S.L.P.; Neves,M.M.; Balarini,M.K.; Rodrigues,M.V.. |
Estudaram-se os efeitos da geleia real sobre a espermatogênese de coelhos tratados com diferentes concentrações de geleia real. Os tratamentos foram formados por três grupos: grupo-controle; grupo que recebeu 0,5mg/dia de geleia real; e grupo que recebeu 1,0mg/dia de geleia real. O estudo envolveu a morfometria testicular. Não houve diferença entre os tratamentos quanto aos pesos corporal (T1=3,20±0,19kg, T2=2,96±0,30kg e T3=3,21±0,37kg) e gonadal (T1=2,36±0,33g, T2=2,53±0,33g e T3=2,64±0,39g) e quanto aos índices gonadossomático (T1=0,15±0,02%, T2=0,17±0,03% e T3=0,16±0,02%) e tubulossomático (T1=0,06±0,01%; T2=0,07±0,01% e T3=0,06±0,01%). O diâmetro médio dos túbulos seminíferos (T1=225,95±13,27µm, T2=239,68±21,50µm e T3=231,57±15,94µm), a altura do... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coelho; Espermatogênese; Compartimento tubular; Túbulos seminíferos; Epitélio seminífero. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400004 |
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Morais,A.C.T.; Barbosa,L.P.; Neves,M.M.; Matta,S.L.P.; Morais,D.B.; Melo,B.E.S.. |
Avaliaram-se os efeitos da geleia real sobre os parâmetros morfofisiológicos testiculares de camundongos (Mus musculus). Utilizaram-se 57 machos Swiss, com quatro meses de idade, distribuídos aleatoriamente em seis tratamentos: T1: solução fisiológica, via intraperitoneal; T2: 0,1mg de geleia real, via intraperitoneal; T3: 0,2mg de geleia real, via intraperitoneal; T4: água destilada, via oral; T5: 0,1mg de geleia real, via oral; e T6: 0,2mg de geleia real, via oral. Após 45 dias de suplementação com geleia real, os animais sacrificados e pesados tiveram seus testículos coletados, incluídos em parafina e corados com hematoxilina/eosina. Não houve diferença entre os tratamentos quanto aos: pesos corporal e testicular, índice gonadossomático, diâmetro... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Camundongo; Mus musculus; Geleia real; Testículo; Morfometria. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100016 |
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Salmito-Vanderley,C.S.B.; Neves,M.M.; Santana,C.V.; Marques Jr.,A.P.; Vasconcelos,A.C.; Nunes,J.E.S.. |
The preservation of the goat genital organs with a fixed solution of paraformaldehyde 10% and a saturated solution of Bouin during 4, 8, 12 and 24 hours of fixation, included in paraffin and stained with hematoxylin-eosin was evaluated. The Bouin solution with four hours of fixation showed to be the best protocol of fixation for the ovary, oviduct, uterus and vagina, resulting in little tissue retraction and better cellular integration when compared to the other fixing times (8, 12 and 24 hours). The fixation with paraformaldehyde showed significant alteration in the tissue architecture of the oviduct and vagina. Althouh paraformaldehyde is not the most adequate solution for the goat genital system preservation, its use can be considered in the absence of... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cabra; Genitália; Solução fixadora; Microscopia de luz. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000100015 |
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Neves,M.M.; Morais,D.B.; Barbosa,L.P.; Gomes,M.G.; Balarine,M.K.. |
The preservation of chinchilla genital organs using a fixed solution of paraformaldehyde 10% buffered and a saturated solution of Bouin during 4, 12 and 18 hours of fixation, inclusion in paraffin and staining with hematoxylin-eosin was evaluated. The Bouin solution with 12 hours of fixation was the best protocol of fixation for the ovaries, oviduct, uterus and vagina, resulting in little tissue retraction and better cellular integration when compared to the other fixing times (4 and 18 hours). The fixation with paraformaldehyde showed good results of tissues fixation. Chinchilla genital organs can be preserved with paraformaldehyde 10% buffered and Bouin solution using 12 hours of fixation. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chinchila; Chinchilla lanigera; Sistema genital; Fêmea; Fixador; Microscopia de luz. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600042 |
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Neves,M.M.; Marques Jr.,A.P.. |
Volumetric proportions and nuclear diameter of the small and large luteinic cells of the corpus luteum (CL) were evaluated in ovaries of 17 Nelore cows and heifers, collected in slaughterhouse and classified into two groups: group I (GI, n=7), non-pregnant animals, and group II (GII, n=10), pregnant animals. The CL was reduced to small cuts (less than 3mm), which were fixed in Bouin solution and processed for morphometric analysis. The volumetric proportion analysis showed higher mean in the GI animals for the nuclei and cytoplasm of luteinic cells, while the mean of connective tissue and fibroblasts was higher in the GII animals, while the mean of the capillary endothelial cells and pericytes did not differ between the groups. The average nuclear diameter... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bovino; Nelore; Corpo lúteo; Morfometria. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500034 |
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Campos,P.K.A.; Barbosa,L.P.; Neves,M.M.; Melo,B.E.S.; Morais,A.C.T.; Morais,D.B.. |
Objetivou-se avaliar o efeito da geleia real na qualidade seminal e na morfometria testicular de coelhos. Quatorze coelhos adultos da raça Nova Zelândia foram distribuídos em três grupos: com administração diária de 1mL de água, via oral (SG); administração diária de 0,5mg (0,5G) de geleia real diluída em 1mL de água, via oral; e administração diária de 1,0mL (1,0G) de geleia real diluída em 1mL de água, via oral. O fornecimento de geleia real foi iniciado 30 dias antes das coletas de sêmen, permanecendo durante todo o período de coleta, totalizando 90 dias. Utilizou-se o método da vagina artificial para coleta de sêmen. Foram avaliados os parâmetros físicos e morfológicos do sêmen e os parâmetros de morfometria testicular. Houve diferença no volume... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Índice gonadossomático; Patologia espermática; Sêmen. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600023 |
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